| Registro completo |
| Provedor de dados: |
55
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| País: |
Brazil
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| Título: |
Detection and molecular characterization of β-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador
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| Autores: |
Delgado,Diana Yessenia Calva
Barrigas,Zorayda Patricia Toledo
Astutillo,Sofía Genoveva Ochoa
Jaramillo,Ana Paulina Arévalo
Ausili,Alessio
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| Data: |
2016-12-01
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| Ano: |
2016
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| Palavras-chave: |
Β-Lactam resistance
Β-Lactamase
ESBL
Genotypic characterization
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| Resumo: |
ABSTRACT This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of β-lactamases and ESBLs with blaTEM and blaCTX-M as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different β-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 β-lactamase and the presence of different CTX-M variants with a prevalence of CTX-M-15. Two infrequent CTX-M variants in South America were also identified. To the best of our knowledge, this is one of the first studies describing the genetic characteristics of β-lactamases in Ecuador.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600627
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2016.07.001
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.20 n.6 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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