| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates
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| Autores: |
Gharout-Sait,Alima
Touati,Abdelaziz
Guillard,Thomas
Brasme,Lucien
Champs,Christophe de
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| Data: |
2015-04-01
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| Ano: |
2015
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| Palavras-chave: |
Enterobacteriaceae
AmpC β-lactamases
Genotyping
Algeria
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| Resumo: |
In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates.Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4- producing isolates and one DHA-1-producing Klebsiella pneumoniae . All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 β-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicates that blaCMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with blaDHA-1. All isolates carrying blaCMY-4 displayed the transposon-like structures ISEcp1/AISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates inAlgeria.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200187
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2014.12.001
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.19 n.2 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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