| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex
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| Autores: |
Martins,Marilena dos Anjos
Brighente,Kate Bastos Santos
Matos,Terezinha Aparecida de
Vidal,Jose Ernesto
Hipólito,Daise Damaris Carnietto de
Pereira-Chioccola,Vera Lucia
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| Data: |
2015-02-01
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| Ano: |
2015
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| Palavras-chave: |
Cryptococcal meningitis
Acquired immunodeficiency syndrome
Polymerase chain reaction
Cerebrospinal fluid
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| Resumo: |
Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal fluid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattiiwas observed in the clinical samples of either group. The 695 bp products of C. neoformanswere observed only in 64.6% of the cryptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The specificity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets.Conclusions:These data suggest that the CN4/CN5 primer set was highly sensitive for the identification of C. neoformans/C. gattiispecies complex in cerebrospinal fluid samples from patients with clinical suspicion of cryptococcal meningitis.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100062
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2014.09.004
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.19 n.1 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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