| Registro completo |
| Provedor de dados: |
BJID
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| País: |
Brazil
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| Título: |
Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy
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| Autores: |
Arrym,Mauro Pedromonico
Alves,Paulo César Martins
Castelhano,Mariana Virginello
Mazzola,Taís Nitsch
Lemos,Renata Muller Banzato Pinto de
Zaccariotto,Tânia Regina
Levy,Carlos Emilio
Guimarães,Fernando
Silva,Marcos Tadeu Nolasco da
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| Data: |
2019-06-01
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| Ano: |
2019
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| Palavras-chave: |
HIV/Aids
BCG
Youth
Tuberculosis
Citotoxicity
Immunologic
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| Resumo: |
ABSTRACT Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. Results: TCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group. Conclusions: Similar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300151
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1016/j.bjid.2019.06.002
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.23 n.3 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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