| Registro completo |
| Provedor de dados: |
BJMBR
|
| País: |
Brazil
|
| Título: |
Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis
|
| Data: |
1997-03-01
|
| Ano: |
1997
|
| Palavras-chave: |
Tetracycline
Mutation
LacZ-fusion
Proteus mirabilis
Bacteriophage Mu
|
| Resumo: |
The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300009
|
| Editor: |
Associação Brasileira de Divulgação Científica
|
| Relação: |
10.1590/S0100-879X1997000300009
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Medical and Biological Research v.30 n.3 1997
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
