| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Immune response to respiratory syncytial virus in young Brazilian children
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| Autores: |
Queiróz,D.A.O.
Durigon,E.L.
Botosso,V.F.
Ejzemberg,B.
Vieira,S.E.
Mineo,J.R.
Yamashita,C.
Hein,N.
Lopes,C.L.
Cacharo,A.L.
Stewien,K.E.
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| Data: |
2002-10-01
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| Ano: |
2002
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| Palavras-chave: |
Respiratory syncytial virus
Peripheral blood mononuclear cells
Young children
Surface antigens
Cytokines
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| Resumo: |
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X2002001000011
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.35 n.10 2002
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| Direitos: |
info:eu-repo/semantics/openAccess
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