Registro completo |
Provedor de dados: |
BJMBR
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País: |
Brazil
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Título: |
Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
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Autores: |
Cui,Xin
Sun,Zheng-Rong
Ren,Gao-Wei
Wang,Gui-Li
Qi,Ying
Ma,Yan-Ping
Ruan,Qiang
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Data: |
2011-12-01
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Ano: |
2011
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Palavras-chave: |
HCMV
UL136
ATPase
Yeast two hybridization
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Resumo: |
Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b’) present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b’ genes may determine the manifestations of HCMV infection in vivo. However, little is known about the host factors, which interact with UL/b’ proteins. This study was conducted to investigate the function of the HCMV UL136 protein. By yeast two-hybrid screening, the β1 subunit of the host Na+/K+-ATPase (ATP1B1) was identified to be a candidate protein, which interacts with the HCMV UL136 protein. The interaction was further evaluated both in vitro by pull-down assay and in vivo by immunofluorescent co-localization. The results showed that the UL136 protein can interact with ATP1B1 in vitro. Co-localization of UL136-EGFP and ATP1B1-DsRed in cell membranes suggests that ATP1B1 was a partner of the UL136 protein. It can be proposed that the HCMV UL136 protein may have important roles in processes such as cell-to-cell spread, and in maintaining cell osmotic pressure and intracellular ion homeostasis during HCMV infection.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200008
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Editor: |
Associação Brasileira de Divulgação Científica
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Relação: |
10.1590/S0100-879X2011007500144
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Medical and Biological Research v.44 n.12 2011
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Direitos: |
info:eu-repo/semantics/openAccess
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