| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Typing class I HLA-A gene using a nested PCR-RFLP procedure
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| Autores: |
Castelli,E.C.
Gil,D.S.
Veiga,L.C.S.
de Camargo,J.L.V.
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| Data: |
2005-06-01
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| Ano: |
2005
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| Palavras-chave: |
Human leukocyte antigen-A
HLA-A
Typing
RFLP
Polymorphism
Major histocompatibility complex
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| Resumo: |
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
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| Tipo: |
Info:eu-repo/semantics/other
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X2005000600004
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.38 n.6 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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