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Provedor de dados:  BJMBR
País:  Brazil
Título:  Tumor necrosis factor alpha increases epithelial barrier permeability by disrupting tight junctions in Caco-2 cells
Autores:  Cui,W.
Li,L.X.
Sun,C.M.
Wen,Y.
Zhou,Y.
Dong,Y.L.
Liu,P.
Data:  2010-04-01
Ano:  2010
Palavras-chave:  TNF-α
Intestinal epithelial barrier
Occludin
Caco-2 cells
Transepithelial permeability
Resumo:  The objectives of this study were to determine the effect of tumor necrosis factor alpha (TNF-α) on intestinal epithelial cell permeability and the expression of tight junction proteins. Caco-2 cells were plated onto Transwell® microporous filters and treated with TNF-α (10 or 100 ng/mL) for 0, 4, 8, 16, or 24 h. The transepithelial electrical resistance and the mucosal-to-serosal flux rates of the established paracellular marker Lucifer yellow were measured in filter-grown monolayers of Caco-2 intestinal cells. The localization and expression of the tight junction protein occludin were detected by immunofluorescence and Western blot analysis, respectively. SYBR-Green-based real-time PCR was used to measure the expression of occludin mRNA. TNF-α treatment produced concentration- and time-dependent decreases in Caco-2 transepithelial resistance and increases in transepithelial permeability to the paracellular marker Lucifer yellow. Western blot results indicated that TNF-α decreased the expression of phosphorylated occludin in detergent-insoluble fractions but did not affect the expression of non-phosphorylated occludin protein. Real-time RT-PCR data showed that TNF-α did not affect the expression of occludin mRNA. Taken together, our data demonstrate that TNF-α increases Caco-2 monolayer permeability, decreases occludin protein expression and disturbs intercellular junctions.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400002
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/S0100-879X2010007500020
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.43 n.4 2010
Direitos:  info:eu-repo/semantics/openAccess
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