Registro completo |
Provedor de dados: |
BJMBR
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País: |
Brazil
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Título: |
Tumor necrosis factor alpha increases epithelial barrier permeability by disrupting tight junctions in Caco-2 cells
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Autores: |
Cui,W.
Li,L.X.
Sun,C.M.
Wen,Y.
Zhou,Y.
Dong,Y.L.
Liu,P.
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Data: |
2010-04-01
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Ano: |
2010
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Palavras-chave: |
TNF-α
Intestinal epithelial barrier
Occludin
Caco-2 cells
Transepithelial permeability
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Resumo: |
The objectives of this study were to determine the effect of tumor necrosis factor alpha (TNF-α) on intestinal epithelial cell permeability and the expression of tight junction proteins. Caco-2 cells were plated onto Transwell® microporous filters and treated with TNF-α (10 or 100 ng/mL) for 0, 4, 8, 16, or 24 h. The transepithelial electrical resistance and the mucosal-to-serosal flux rates of the established paracellular marker Lucifer yellow were measured in filter-grown monolayers of Caco-2 intestinal cells. The localization and expression of the tight junction protein occludin were detected by immunofluorescence and Western blot analysis, respectively. SYBR-Green-based real-time PCR was used to measure the expression of occludin mRNA. TNF-α treatment produced concentration- and time-dependent decreases in Caco-2 transepithelial resistance and increases in transepithelial permeability to the paracellular marker Lucifer yellow. Western blot results indicated that TNF-α decreased the expression of phosphorylated occludin in detergent-insoluble fractions but did not affect the expression of non-phosphorylated occludin protein. Real-time RT-PCR data showed that TNF-α did not affect the expression of occludin mRNA. Taken together, our data demonstrate that TNF-α increases Caco-2 monolayer permeability, decreases occludin protein expression and disturbs intercellular junctions.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400002
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Editor: |
Associação Brasileira de Divulgação Científica
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Relação: |
10.1590/S0100-879X2010007500020
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Medical and Biological Research v.43 n.4 2010
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Direitos: |
info:eu-repo/semantics/openAccess
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