| Registro completo |
| Provedor de dados: |
56
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| País: |
Brazil
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| Título: |
Serotonin regulates osteoblast proliferation and function in vitro
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| Autores: |
Dai,S.Q.
Yu,L.P.
Shi,X.
Wu,H.
Shao,P.
Yin,G.Y.
Wei,Y.Z.
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| Data: |
2014-09-01
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| Ano: |
2014
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| Palavras-chave: |
Serotonin
Osteoblast
Proliferation
Differentiation
5-HT receptors
5-HT
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| Resumo: |
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and mineralization at low concentrations, but this inhibitory effect was mitigated at relatively high concentrations. Six of the 5-HT receptor subtypes (5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A, 5-HT2B, and 5-HT2C) were found to exist in rat osteoblasts. Of these, 5-HT2A and 5-HT1Breceptors had the highest expression levels, at both early and late stages of differentiation. Our results indicated that 5-HT can regulate osteoblast proliferation and function in vitro.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/1414-431X20143565
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.47 n.9 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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