| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis
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| Autores: |
Zhang,You En
Huang,Guang Qing
Wu,Bing
Lin,Xin Duo
Yang,Wen Zi
Ke,Zun Yu
Liu,Jie
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| Data: |
2019-01-01
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| Ano: |
2019
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| Palavras-chave: |
Reactive oxygen species
ROS
Hydrogen sulfide
H2O2-induced apoptosis
Cardiomyocytes
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| Resumo: |
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400608
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/1414-431x20187626
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.52 n.4 2019
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| Direitos: |
info:eu-repo/semantics/openAccess
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