| Registro completo |
| Provedor de dados: |
56
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| País: |
Brazil
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| Título: |
Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion
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| Autores: |
Silveira,N.A.
Franci,C.R.
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| Data: |
1999-09-01
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| Ano: |
1999
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| Palavras-chave: |
Neuropeptide Y
Medial preoptic area
Neuropeptide Y-Y1 receptor
Prolactin
MRNA for neuropeptide
Y-Y1 receptor
Luteinizing hormone
Follicle-stimulating hormone
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| Resumo: |
We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 ± 5.9 ng/ml in the sense group to 52.9 ± 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean ± SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900016
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X1999000900016
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.32 n.9 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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