| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Electrophysiological evidence for glial-subtype glutamate transporter functional expression in rat cerebellar granule neurons
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| Autores: |
Mafra,R.A.
Leão,R.M.
Beirão,P.S.L.
Cruz,J.S.
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| Data: |
2003-07-01
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| Ano: |
2003
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| Palavras-chave: |
Cerebellar granule neurons
Excitatory amino-acid transporter type 2
Patch-clamp
Glutamate
Whole-cell voltage-clamp
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| Resumo: |
A glutamate-sensitive inward current (Iglu) is described in rat cerebellar granule neurons and related to a glutamate transport mechanism. We examined the features of Iglu using the patch-clamp technique. In steady-state conditions the Iglu measured 8.14 ± 1.9 pA. Iglu was identified as a voltage-dependent inward current showing a strong rectification at positive potentials. L-Glutamate activated the inward current in a dose-dependent manner, with a half-maximal effect at about 18 µM and a maximum increase of 51.2 ± 4.4%. The inward current was blocked by the presence of dihydrokainate (0.5 mM), shown by others to readily block the GLT1 isoform. We thus speculate that Iglu could be attributed to the presence of a native glutamate transporter in cerebellar granule neurons.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700018
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X2003000700018
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.36 n.7 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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