| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Increase of stress resistance in Lactococcus lactis via a novel food-grade vector expressing a shsp gene from Streptococcus thermophilus
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| Autores: |
Tian,Hongtao
Tan,Jianxin
Zhang,Lifang
Gu,Xinxi
Xu,Wentao
Guo,Xinghua
Luo,Yunbo
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| Data: |
2012-09-01
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| Ano: |
2012
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| Palavras-chave: |
Shsp gene
Streptococcus thermophilus
Lactococcus lactis
Food-grade vector
Stress resistance
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| Resumo: |
The effects of the expression of a small heat shock protein (shsp) gene from Streptococcus thermophilus on stress resistance in Lactococcus lactis under different environmental stresses were investigated in this study. pMG36e-shsp, an expression vector, was first constructed by inserting a shsp open reading frame (ORF) cloned from S. thermophilus strain St-QC into pMG36e. Then, a food-grade expression vector, pMG-shsp, was generated by deleting the erythromycin resistance gene from pMG36e-shsp. The transformation rate of pMG-shsp was comparable to that of pMG36e-shsp when each of these two vectors was introduced into L. lactis. These results demonstrated that the shsp ORF could successfully used as a food-grade selection marker in both pMG-shsp and pMG36e-shsp. Furthermore, the growth characteristics were almost the same between L. lactis ML23 transformants harboring pMG36e or pMG-shsp. The survival rate of L. lactis ML23 expressing the shsp ORF were increased to 0.032%, 0.006%, 0.0027%, 0.03%, and 0.16% under the following environmental stresses: heat, acid, ethanol, bile salt and H2O2, respectively. These results indicated that the expression of the shsp gene in the food-grade vector pMG-shsp conferred resistance to environmental stresses without affecting the growth characteristics of L. lactis ML23.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300043
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822012000300043
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.43 n.3 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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