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Provedor de dados:  BJM
País:  Brazil
Título:  Is Malassezia nana the main species in horses’ ear canal microbiome?
Autores:  Aldrovandi,Ana Lúcia
Osugui,Lika
Coutinho,Selene Dall’ Acqua
Data:  2016-09-01
Ano:  2016
Palavras-chave:  Malassezia nana
Malassezia slooffiae
Microbiome
Horses
Ear canal
Resumo:  ABSTRACT The objective of this study was to characterize genotypically Malassezia spp. isolated from the external ear canal of healthy horses. Fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. External ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. A total of 110 samples were cultured into Dixon medium and were incubated at 32 °C for up to 15 days. Macro- and micromorphology and phenotypic identification were performed. DNA was extracted, strains were submitted to polymerase chain reaction technique, and the products obtained were submitted to Restriction Fragment Length Polymorphism using the restriction enzymes BstCI and HhaI. Strains were sent off to genetic sequencing of the regions 26S rDNA D1/D2 and ITS1-5.8S-ITS2 rDNA. Malassezia spp. were isolated from 33/55 (60%) animals and 52/110 (47%) ear canals. No growth on Sabouraud dextrose agar was observed, confirming the lipid dependence of all strains. Polymerase chain reaction-Restriction fragment length polymorphism permitted the molecular identification of Malassezia nana - 42/52 (81%) and Malassezia slooffiae - 10/52 (19%). Sequencing confirmed RFLP identification. It was surprising that M. nana represented over 80% of the strains and no Malassezia equina was isolated in this study, differing from what was expected.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300770
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2016.04.017
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.47 n.3 2016
Direitos:  info:eu-repo/semantics/openAccess
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