| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Zika detection: comparison of methodologies
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| Autores: |
Colombo,Tatiana Elias
Terzian,Ana Carolina Bernardes
Araújo Júnior,João Pessoa
Parreira,Ricardo
Cabrera,Eliana Márcia Sotello
Santos,Izalco Nuremberg Penha dos
Reis,Andréia Francesli Negri
Costa,Fabiana Rodrigues
Cruz,Lilian Elisa Arão Antônio
Rombola,Patrícia Lopes
Nogueira,Maurício Lacerda
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| Data: |
2018-03-01
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| Ano: |
2018
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| Palavras-chave: |
Zika virus
Detection
Sensitivity
Specificity
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| Resumo: |
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100144
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2017.04.011
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.49 n.1 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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