| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Kinetic properties of Streptomyces canarius L- Glutaminase and its anticancer efficiency
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| Autores: |
Reda,Fifi M.
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| Data: |
2015-12-01
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| Ano: |
2015
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| Palavras-chave: |
L-glutaminase
Anti-cancer
Cytotoxicity
MTT assay
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| Resumo: |
Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets after 10 days of rabbit's injection. The anticancer activity of L-glutaminase was also tested against five types of human cancer cell lines using MTT assay in vitro. L-glutaminase has a significant efficiency against Hep-G2 cell (IC50, 6.8 μg/mL) and HeLa cells (IC50, 8.3 μg/mL), while the growth of MCF-7 cells was not affected. L-glutaminase has a moderate cytotoxic effect against HCT-116 cell (IC50, 64.7 μg/mL) and RAW 264.7 cell (IC50, 59.3 μg/mL).
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400957
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-838246420130847
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.46 n.4 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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