| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity
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| Autores: |
Gong,Tianxiang
Li,Wanyi
Wang,Yueling
Jiang,Yan
Zhang,Qiang
Feng,Wei
Jiang,Zhonghua
Li,Mingyuan
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| Data: |
2011-09-01
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| Ano: |
2011
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| Palavras-chave: |
Mouse beta defensin 2
Expression
Purification
Antimicrobial activity
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| Resumo: |
Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters: induce with 0.6 mM isopropylthiogalactoside (IPTG) at 34ºC in 2×YT medium and harvest at 6 h postinduction, fusion protein TrxA-mBD2 was high expressed in the soluble fraction (>95%). After cleaved fusion protein by enterokinase, soluble mature mBD2 was achieved 6 mg/L with a volumetric productivity. Purified recombinant mBD2 demonstrated clear broad-spectrum antimicrobial activity for fungi, bacteria and virus. The MIC of antibacterial activity of against Staphylococcus aureus was 50 µg/ml. The MIC of against Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans) was 12.5µg/ml and 25µg/ml, respectively. Also, the antimicrobial activity of mBD2 was effected by NaCl concentration. Additionally, mBD2 showed antiviral activity against influenza A virus (IAV), the protective rate for Madin-Darby canine kidney cells (MDCK) was 93.86% at the mBD2 concentration of 100 µg/ml. These works might provide a foundation for the following research on the mBD2 as therapeutic agent for medical microbes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300043
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822011000300043
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.42 n.3 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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