| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Optimization of growth medium for protease production by Haloferax Lucentensis VKMM 007 by response surface methodology
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| Autores: |
Manikandan,Muthu
Kannan,Vijayaraghavan
Velikonja,Blagajana Herzog
Paic,Lejla
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| Data: |
2011-06-01
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| Ano: |
2011
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| Palavras-chave: |
Cultivation media
Haloferax lucentensis
Response surface methodology
Optimization
Halophilic protease
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| Resumo: |
The production of halophilic thermostable protease by Haloferax lucentensis VKMM 007 was optimized using a statistical approach. In accordance with factorial design, soluble starch, gelatin, KCl and MgSO4 were selected among 27 variables tested. Next, a second-order quadratic model was estimated and optimal medium concentrations were determined based on quadratic regression equation generated by model. These were 5.14 g L-1 of KCl, 6.57 g L-1of MgSO4, 9.05 g L-1of gelatin and 5.27 g L-1of soluble starch in high salts media supplemented with 0.5% (w/v) of beef extract and peptone, respectively. In these optimal conditions, the obtained protease concentration of 6.80 U mL-1 was in agreement with the predicted protease concentration and was further improved to 7.02 U mL-1 by increasing the concentration of NaCl in the medium to 25% (w/v). An overall 4.0-fold increase in protease production was achieved in the optimized medium compared to activity obtained in initial medium.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200050
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822011000200050
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.42 n.2 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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