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Provedor de dados:  BJM
País:  Brazil
Título:  Selection of endophytic fungi from comfrey (Symphytum officinale L.) for in vitro biological control of the phytopathogen Sclerotinia sclerotiorum (Lib.)
Autores:  Rocha,Rafaeli
Luz,Daniela Eleutério da
Engels,Cibelle
Pileggi,Sônia Alvim Veiga
Jaccoud Filho,David de Souza
Matiello,Rodrigo Rodrigues
Pileggi,Marcos
Data:  2009-03-01
Ano:  2009
Palavras-chave:  Biological control
Sclerotinia sclerotiorum (Lib.)
Endophytic strain
Symphytum officinale L
Resumo:  Biological control consists of using one organism to attack another that may cause economic damage to crops. Integrated Pest Management (IPM) is a very common strategy. The white mold produced by Sclerotiniasclerotiorum (Lib.) causes considerable damage to bean crops. This fungus is a soil inhabitant, the symptoms of which are characterized by water-soaked lesions covered by a white cottony fungal growth on the soil surface and/or the host plant. Possible biological control agents taken from plants are being investigated as phytopathogen inhibitors. These are endophytic microorganisms that inhabit the intercellular spaces of vegetal tissues and are often responsible for antimicrobial production. The objective of the present study was to select endophytic fungi isolated from comfrey (Symphytumofficinale L.) leaves with in vitro antagonist potential against the phytopathogenic fungus S. sclerotiorum. Twelve isolates of endophytic fungi and a pathogenic strain of S. sclerotiorum were used in the challenge method. With the aid of this method, four endophytes with the best antagonistic activity against S. sclerotiorum were selected. Pathogen growth inhibition zones were considered indicative of antibiosis. The percentages of pathogenic mycelia growth were measured both with and without the antagonist, resulting in growth reductions of 46.7% to 50.0% for S. sclerotiorum. These analyses were performed by evaluating the endophytic/pathogenic mycelia growth in mm/day over an eight-day period of antagonistic tests.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100011
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822009000100011
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.40 n.1 2009
Direitos:  info:eu-repo/semantics/openAccess
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