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Provedor de dados:  BJM
País:  Brazil
Título:  Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions
Autores:  Monteiro,Francielle Liz
Cargnelutti,Juliana Felipetto
Martins,Mathias
Anziliero,Deniz
Erhardt,Magnólia Martins
Weiblen,Rudi
Flores,Eduardo Furtado
Data:  2016-12-01
Ano:  2016
Palavras-chave:  CDV
CPIV
CAdV-2
CaHV-1
Resumo:  Abstract Three dog shelters in Rio Grande do Sul were investigated for associations between the occurrence of respiratory viruses and shelter environmental conditions. Nasal secretions randomly collected during the cold season were tested via PCR, and this data collection was followed by nucleotide sequencing of the amplicons. In shelter #1 (poor sanitary and nutritional conditions, high animal density and constant contact between dogs), 78% (58/74) of the nasal samples were positive, 35% (26/74) of which were in single infections and 44% (32/74) of which were in coinfections. Shelters #2 and #3 had satisfactory sanitary and nutritional conditions, outdoors exercise areas (#2) and animal clustering by groups (#3). In shelter #2, 9% (3/35) of the samples were positive for Canine parainfluenza virus (CPIV), and 6% (2/35) were positive for Canid herpesvirus 1 (CaHV-1). In shelter #3, 9% (7/77) of the samples were positive for Canine adenovirus type 2 (CAdV-2), and 1% (1/77) were positive for Canine distemper virus (CDV). The amplicon sequences (CPIV and CDV nucleoprotein gene; CAdV-2 E3 gene; CaHV-1 glycoprotein B gene) showed 94-100% nucleotide identity with GenBank sequences. Our results demonstrate that CPIV, CAdV-2 and CDV are common in dog shelters and that their frequencies appear to be related with environmental and nutritional conditions. These results indicate the need for control/prevention measures, including vaccination and environmental management, to minimize these infections and improve dog health.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400876
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2016.07.002
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.47 n.4 2016
Direitos:  info:eu-repo/semantics/openAccess
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