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Provedor de dados:  BJM
País:  Brazil
Título:  Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov
Autores:  Visnuvinayagam,Sivam
K,Thangavel
N,Lalitha
S,Malmarugan
Sukumar,Kuppannan
Data:  2015-09-01
Ano:  2015
Palavras-chave:  Newcastle disease
Komarov strain
Chicken embryo fibroblast
Newcastle disease vaccine
Resumo:  Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18th(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300861
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246320140051
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.3 2015
Direitos:  info:eu-repo/semantics/openAccess
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