| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
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| Autores: |
Han,Xufeng
Wang,Lei
Li,Wei
Li,Bibo
Yang,Yuxin
Yan,Hailong
Qu,Lei
Chen,Yulin
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| Data: |
2015-09-01
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| Ano: |
2015
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| Palavras-chave: |
Green fluorescent protein
Gastro-intestinal tract
Lactobacillus plantarum
Goat
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| Resumo: |
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-838246320140556
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.46 n.3 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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