| Registro completo |
| Provedor de dados: |
BJM
|
| País: |
Brazil
|
| Título: |
Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system
|
| Autores: |
Dambros,Régia Maria Feltrin
Ribeiro,Bergman Moraes
Aguiar,Raimundo Wagner de S.
Schaefer,Rejane
Esteves,Paulo Augusto
Perecmanis,Simone
Simon,Neide Lisiane
Silva,Nayara Cavalcante
Coldebella,Michele
Ciacci-Zanella,Janice Reis
|
| Data: |
2007-09-01
|
| Ano: |
2007
|
| Palavras-chave: |
Aujeszky
Glycoprotein E
Baculovírus
Recombinant
|
| Resumo: |
Aujeszky' s disease (AD) is an infectious disease causing important economic losses to the swine industry worldwide. The disease is caused by an alpha-herpesvirus, Aujeszky' s disease virus (ADV), an enveloped virus with a double stranded linear DNA genome. The ADV genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to the infection. The glycoprotein E (gE) is a non-essential protein and deletion of the gE gene has been used for the production of marker vaccines. Aiming to develop molecular reagents for the production of a gE specific ELISA test, the gE gene was amplified by PCR, cloned and expressed into a baculovirus expression system. The recombinant DNA vector pFastBac-gE-ADV was used for site-specific transposition into the recombinant baculovirus (bacmid). Colonies with recombinant bacmid-pFastBac-gE-ADV were selected by antibiotic and color selection and the presence of the gE gene was confirmed by PCR. The recombinant bacmid-pFastBac-gE-ADV was cotransfected in insect Trichoplusia ni and the presence of the recombinant DNA and gE protein were detected by PCR, SDS-PAGE and Western blotting, respectively.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300021
|
| Editor: |
Sociedade Brasileira de Microbiologia
|
| Relação: |
10.1590/S1517-83822007000300021
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Microbiology v.38 n.3 2007
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
