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Provedor de dados:  BJM
País:  Brazil
Título:  Identification and pathogenicity of Malassezia species isolated from human healthy skin and with macules
Autores:  Neves,Rejane Pereira
Magalhães,Oliane Maria Correia
Silva,Merivânia Lucas da
Souza-Motta,Cristina Maria de
Queiroz,Lusinete Aciole de
Data:  2005-06-01
Ano:  2005
Palavras-chave:  Malassezia furfur
M. simpodialis
Pathogenicity
Resumo:  This study investigated the ocurrence of Malassezia species in clinically healthy students and with macules with a slight fawn discoloration and characterized the isolates as to the pathogenicity factors such as growth at 37ºC, lipase, phospholipase and protease detection. Clinical samples were collected from different body sites of one hundred students of different ages and both sexes. The samples, obtained by scrapping the skin surface and the scalp, were treated with potassa and cultured. Cultures were obtained in Petri dishes containing Sabouraud agar medium added of olive oil, incubated at room temperature and at 37ºC. Culture identifications were based in their morphological and physiological properties. Lipase, phospholipase and protease detection was performed in specific media on Petri dishes for formation of a zone. Globose, spherical yeast cells and hypha were investigated by direct microscopy of clinical materials. Malassezia furfur was detected in seven samples and M. sympodialis in four. All Malassezia cultures presented lipase activity, but none was phospholipase positive. Protease activity was observed in two M. furfur and two M. simpodialis isolates.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200003
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822005000200003
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.36 n.2 2005
Direitos:  info:eu-repo/semantics/openAccess
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