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Provedor de dados:  58
País:  Brazil
Título:  Mannanoligosaccharide agglutination by Salmonella enterica strains isolated from carrier pigs
Autores:  Borowsky,Luciane
Corção,Gertrudes
Cardoso,Marisa
Data:  2009-09-01
Ano:  2009
Palavras-chave:  Salmonella
Type-1 fimbriae
Mannanoligosaccharides
Resumo:  Type-1 fimbriae are associated with most Salmonella enterica serovars and are an essential factor for host colonization. Mannanoligosaccharides (MOS), a prebiotic that is agglutinated by type-1 fimbriae, are proposed for the control of enterobacteria colonization and may be an alternative to Salmonella control in pigs. The aim of this study was to evaluate the capability of porcine Salmonella strains to adhere to MOS in vitro. A total of 108 strains of Salmonella sp. isolated from carrier pigs were evaluated for the amplification of fimA and fimH genes, agglutination of MOS and hemagglutination. In all tested strains, amplicons of expected size were detected for both fimA and fimH gene. In the hemagglutination assays, 31 (28.7%) strains presented mannose-sensitive agglutination of erythrocytes, indicating that the strains were expressing type-1 fimbriae. Considering only strains expressing the type-1 fimbriae, 23 (74.2%) presented a strong agglutination of MOS, 3 (9.6%) a weak reaction and 5 (16.2%) none. The results indicate that Salmonella enterica strains expressing type-1 fimbriae can agglutinate effectively in vitro to MOS.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300007
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822009000300007
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.40 n.3 2009
Direitos:  info:eu-repo/semantics/openAccess
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