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	Provedor de dados eCrystals (44) Autor Hursthouse, Michael B. (43) Coles, Simon J. (40) Davies, David B. (29) Kilic, A. (27) Uslu, A. (10) Besli, S (8) Besli, S. (6) Light, Mark E. (5) Kilic, Adem (4) Shaw, R.A. (4) Bell, N. A. (3) Constable, C. P. (3) Hibbs, D. E. (3) Huth, Susanne. L. (3) Mansor, R. (3) Mayer, Thomas A. (3) Odlyha, M. (3) Raper, E. S. (3) Rutherford, J.S. (3) Sammon, C. (3) Mais... Palavra-chave Inorganic (44) Cyclophosphazene (7) C~3~H~6~Cl~4~N~3~O~2~P~3~ (3) Phase transition (3) Variable temperature (3) Catalysis (2) Crystal structures (2) Cyclophosphazenes (2) C~14~H~8~HgN~2~S~4~ (2) C~18~H~24~N~5~O~2~P~3~S~2~ (2) C~22~H~32~N~3~O~7~P~3~ (2) C~4~H~8~Cl~4~N~3~O~2~P~3~ (2) C~7~H~14~Cl~4~N~3~O~2~P~3~ (2) C~9~H~18~Cl~4~N~3~O~2~P~3~ (2) Hypercoordination (2) Molecular parameters (2) Phosphonium salts (2) Phosphorus-nitrogen compounds (2) Stibonium salts (2) Substituent basicity constants (2) Mais... Tipo do documento Crystal Structure Data Holding (44) Ano 2008 (13) 2007 (16) 2004 (2) 2002 (4) 2001 (3) 2000 (2) 1997 (3) 1996 (1) Mais... País United Kingdom (44)		Registro completo Provedor de dados:  BJM País:  Brazil Título:  Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis Autores:  Salles,Joana Falcão Gitahy,Patrícia de Medeiros Skøt,Leif Baldani,José Ivo Data:  2000-09-01 Ano:  2000 Palavras-chave:  Gluconacetobacter diazotrophicus Herbaspirillum seropedicae Endophytic bacteria Diazotrophic Cry gene Resumo:  The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Western-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The delta-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the delta-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests. Tipo:  Info:eu-repo/semantics/article Idioma:  Inglês Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001 Editor:  Sociedade Brasileira de Microbiologia Relação:  10.1590/S1517-83822000000300001 Formato:  text/html Fonte:  Brazilian Journal of Microbiology v.31 n.3 2000 Direitos:  info:eu-repo/semantics/openAccess Fechar

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