| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media
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| Autores: |
Paz,Marcelo Fossa da
Baruque-Ramos,Júlia
Hiss,Haroldo
Vicentin,Márcio Alberto
Leal,Maria Betania Batista
Raw,Isaías
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| Data: |
2003-04-01
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| Ano: |
2003
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| Palavras-chave: |
Neisseria meningitidis
Culture medium
Vaccine
Polysaccharide
Batch cultivation
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| Resumo: |
Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and vessel pressure 6 psi, without dissolved oxygen or pH controls. The cultivation runs were divided in three groups, with 3 repetitions each. The cultivation using the Frantz medium presented the best results: average of final polysaccharide concentration = 0.134 g/L and Y P/X=0.121, followed by Catlin 6 medium, with results of 0.095 g/L and 0.067 respectively. Considering the principal advantages in the use of the synthetic medium, i.e. facilitation of a cultivation and purification steps of the polysaccharide production process, there is a possibility that in the near future, Catlin 6 will replace the traditional Frantz medium.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822003000100007
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.34 n.1 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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