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Provedor de dados:  BJM
País:  Brazil
Título:  Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1
Autores:  Veana,F.
Martínez-Hernández,J.L.
Aguilar,C.N.
Rodríguez-Herrera,R.
Michelena,G.
Data:  2014-06-01
Ano:  2014
Palavras-chave:  By-products
Virgin and hydrolyzated bagasse
Β-fructofuranosidase
Xerophilic
Resumo:  Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previously reported as an invertase over-producer strain was used. Molasses from Mexico and Cuba were chemically analyzed (total and reducer sugars, nitrogen and phosphorous contents); the last one was selected based on chemical composition. Fermentations were performed using virgin and hydrolyzate bagasse (treatment with concentrated sulfuric acid). Results indicated that, the enzymatic yield (5231 U/L) is higher than those reported by other A. niger strains under solid state fermentation, using hydrolyzate bagasse. The acid hydrolysis promotes availability of fermentable sugars. In addition, maximum invertase activity was detected at 24 h using low substrate concentration, which may reduce production costs. This study presents an alternative method for invertase production using a xerophilic fungus isolated from Mexican semi-desert and inexpensive substrates (molasses and sugarcane bagasse).
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200002
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822014000200002
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.45 n.2 2014
Direitos:  info:eu-repo/semantics/openAccess
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