| Registro completo |
| Provedor de dados: |
BJM
|
| País: |
Brazil
|
| Título: |
Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production
|
| Autores: |
Carneiro-da-Cunha,Maria das Graças
Lima Filho,José Luiz de
Campos-Takaki,Galba Maria de
|
| Data: |
2002-12-01
|
| Ano: |
2002
|
| Palavras-chave: |
Streptomyces clavuligerus
Protoplast
Clavulanic acid
|
| Resumo: |
Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation and regeneration techniques resulted in a new isolate strain of Streptomyces clavuligerus that produced approximately 2.5 fold more clavulanic acid.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014
|
| Editor: |
Sociedade Brasileira de Microbiologia
|
| Relação: |
10.1590/S1517-83822002000400014
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Microbiology v.33 n.4 2002
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
