| Registro completo |
| Provedor de dados: |
58
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| País: |
Brazil
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| Título: |
Molecular characterization of the first leptospires isolated from goats in Brazil
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| Autores: |
Lilenbaum,Walter
Kremer,Frederico
Ristow,Paula
Dellagostin,Odir
Bourhy,Pascale
Hartskeerl,Rudy
Vasconcellos,Silvio
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| Data: |
2014-12-01
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| Ano: |
2014
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| Palavras-chave: |
Leptospira
Serogrouping
Sequencing
PFGE
Rrs
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| Resumo: |
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822014000400050
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.45 n.4 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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