| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Characterization of pyrene utilizing Bacillus spp. from crude oil contaminated soil
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| Autores: |
Khanna,Purnima
Goyal,Dinesh
Khanna,Sunil
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| Data: |
2012-06-01
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| Ano: |
2012
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| Palavras-chave: |
Pyrene
Bacillus
Crude oil contaminated soil
High performance liquid chromatography (HPLC)
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| Resumo: |
Pyrene, a high molecular weight polycyclic aromatic hydrocarbon (PAH), is a priority pollutant present in soil contaminated with crude oil, coal-tar and complex PAHs. Bacterial consortium CON-3 developed from crude oil contaminated soil of Patiala, Punjab (India) cometabolized 50 µg ml-1 pyrene in the presence of glucose (0.5 %; w/v) at 30 °C, as determined by reverse-phase high performance liquid chromatography (HPLC). Bacillus sp. PK-12, Bacillus sp. PK-13 and Bacillus sp. PK-14 from CON-3, identified by 16S rRNA gene sequence analysis, were able to cometabolize 64 %, 55 % and 53 % of pyrene in 35 days, respectively. With the increase in glucose concentration to 1.0 % (w/v) in growth medium isolates PK-12, PK-13 and PK-14 showed 19 - 46 % uptake of 50 µg ml-1 pyrene in 4 days, respectively. Uptake of pyrene was correlated with growth and biosurfactant activity, which is suggestive of the potential role of members of Bacillus genera in pyrene mobilization and its uptake.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200024
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822012000200024
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.43 n.2 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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