Registro completo |
Provedor de dados: |
BJM
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País: |
Brazil
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Título: |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
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Autores: |
Shabbiri,Khadija
Adnan,Ahmad
Jamil,Sania
Ahmad,Waqar
Noor,Bushra
Rafique,H.M.
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Data: |
2012-09-01
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Ano: |
2012
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Palavras-chave: |
Brevibacterium linens
Protease
Solid-state fermentation
Plackett-Burman Design
Central Composite Design (CCD)
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Resumo: |
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
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Editor: |
Sociedade Brasileira de Microbiologia
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Relação: |
10.1590/S1517-83822012000300031
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Formato: |
text/html
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Fonte: |
Brazilian Journal of Microbiology v.43 n.3 2012
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Direitos: |
info:eu-repo/semantics/openAccess
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