| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells
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| Autores: |
Saliba,Alessandra Mattos
Nishi,Rebeca
Assis,Maria Cristina
Plotkowski,Maria Cristina
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| Data: |
2003-11-01
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| Ano: |
2003
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| Palavras-chave: |
Type III secretion system
ExoU
Endothelial cells
Bacterial cytotoxicity
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| Resumo: |
To ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not interfere with its ability to kill host cells.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822003000500008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.34 suppl.1 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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