| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis
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| Autores: |
Koga,Paula Célia Mariko
Menezes,Caroline Anunciação
Lima,Flávia Afonso
Nara,Júlia Mitico
Magalhães,Caroline Arantes
Cianciarullo,Aurora Marques
Ferreira-Júnior,Jorge Mario da Costa
Trabulsi,Luiz Rachid
Mendes-Ledesma,Meire Roberta Bresciani
Piazza,Roxane Maria Fontes
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| Data: |
2003-11-01
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| Ano: |
2003
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| Palavras-chave: |
Intimin
Phenotypical analysis
Polyclonal antibody
EPEC
EHEC
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| Resumo: |
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822003000500002
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.34 suppl.1 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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