| Registro completo |
| Provedor de dados: |
BJM
|
| País: |
Brazil
|
| Título: |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68
|
| Autores: |
Aygan,Ashabil
Arikan,Burhan
Korkmaz,Hatice
Dinçer,Sadik
Çolak,Ömer
|
| Data: |
2008-09-01
|
| Ano: |
2008
|
| Palavras-chave: |
Bacillus sp.
Α-amylase
Alkaliphilic
Thermostable
Enzyme
|
| Resumo: |
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by SDS and EDTA by 38% and 34%, respectively.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027
|
| Editor: |
Sociedade Brasileira de Microbiologia
|
| Relação: |
10.1590/S1517-83822008000300027
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Microbiology v.39 n.3 2008
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
