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Provedor de dados:  58
País:  Brazil
Título:  Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
Autores:  Jiang,Yun-Gang
Wang,Chu-Yan
Jin,Chao
Jia,Jun-Qiang
Guo,Xijie
Zhang,Guo-Zheng
Gui,Zhong-Zheng
Data:  2014-06-01
Ano:  2014
Palavras-chave:  Mulberry
DNJ Production
Microorganisms
Fermentation
Resumo:  DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822014000200048
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.45 n.2 2014
Direitos:  info:eu-repo/semantics/openAccess
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