| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Culture conditions for the production of thermostable amylase by Bacillus sp
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| Autores: |
Teodoro,Carlos Eduardo de Souza
Martins,Meire Lelis Leal
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| Data: |
2000-10-01
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| Ano: |
2000
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| Palavras-chave: |
Alpha-amylase
Thermophilic bacterium
Thermostable enzymes
Bacillus sp
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| Resumo: |
Studies on the alpha-amylase production were carried out with a bacterial strain isolated from a soil sample. The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. The optimal pH and temperature for activity were 6.0 and 50°C respectively. The enzyme extract retained 100% activity when incubated for one hour at 90°C and 40% at 60°C for 24 h. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400011
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822000000400011
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.31 n.4 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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