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Provedor de dados:  BJM
País:  Brazil
Título:  Comparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis
Autores:  Melo,Poliana de Castro
Ferreira,Luciano Menezes
Nader Filho,Antônio
Zafalon,Luiz Francisco
Vicente,Hinig Isa Godoy
Souza,Viviane de
Data:  2013-01-01
Ano:  2013
Palavras-chave:  Biofilms
Bovine mastitis
Phenotypic and molecular analysis
Staphylococcus aureus
Resumo:  Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822013005000031
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.44 n.1 2013
Direitos:  info:eu-repo/semantics/openAccess

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