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Provedor de dados:  BJM
País:  Brazil
Título:  Inactivation of MarR gene homologs increases susceptibility to antimicrobials in Bacteroides fragilis
Autores:  Silva,Clara Maria Guimarães
Silva,Déborah Nascimento dos Santos
Costa,Scarlathe Bezerra da
Almeida,Juliana Soares de Sá
Boente,Renata Ferreira
Teixeira,Felipe Lopes
Domingues,Regina Maria Cavalcanti Pilotto
Lobo,Leandro Araujo
Data:  2018-03-01
Ano:  2018
Palavras-chave:  Bacteroides fragilis
Anaerobic bacteria
Multi-drug resistance
Oxidative stress resistance
Resumo:  ABSTRACT Bacteroides fragilis is the strict anaerobic bacteria most commonly found in human infections, and has a high mortality rate. Among other virulence factors, the remarkable ability to acquire resistance to a variety of antimicrobial agents and to tolerate nanomolar concentrations of oxygen explains in part their success in causing infection and colonizing the mucosa. Much attention has been given to genes related to multiple drug resistance derived from plasmids, integrons or transposon, but such genes are also detected in chromosomal systems, like the mar (multiple antibiotic resistance) locus, that confer resistance to a range of drugs. Regulators like MarR, that control expression of the locus mar, also regulate resistance to organic solvents, disinfectants and oxygen reactive species are important players in these events. Strains derived from the parental strain 638R, with mutations in the genes hereby known as marRI (BF638R_3159) and marRII (BF638R_3706) were constructed by gene disruption using a suicide plasmid. Phenotypic response of the mutant strains to hydrogen peroxide, cell survival assay against exposure to oxygen, biofilm formation, resistance to bile salts and resistance to antibiotics was evaluated. The results showed that the mutant strains exhibit statistically significant differences in their response to oxygen stress, but no changes were observed in survival when exposed to bile salts. Biofilm formation was not affected by either gene disruption. Both mutant strains however, became more sensitive to multiple antimicrobial drugs tested. This indicates that as observed in other bacterial species, MarR are an important resistance mechanism in B. fragilis.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100200
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2017.05.005
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.49 n.1 2018
Direitos:  info:eu-repo/semantics/openAccess
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