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Provedor de dados:  BJM
País:  Brazil
Título:  Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods
Autores:  Cunha,Maria de Lourdes Ribeiro de Souza da
Peresi,Eliana
Calsolari,Regina Adriana Oliveira
Araújo Júnior,João Pessoa
Data:  2006-03-01
Ano:  2006
Palavras-chave:  Coagulase-negative staphylococci
PCR
Enterotoxins
Resumo:  Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the isolates (40%). Further isolates included S. xylosus (20%), S. warneri (20%), S. saccharolyticus (15%), and S. hominis (5%). Four isolates were positive for enterotoxin genes, as detected by polymerase chain reaction, with sea being the predominant gene. Although no enterotoxin production was detected by the reverse passive latex agglutination method, the data showed that the toxigenic capacity of CNS should not be ignored, requiring investigation of this group of microorganisms in food.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822006000100013
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.37 n.1 2006
Direitos:  info:eu-repo/semantics/openAccess
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