| Registro completo |
| Provedor de dados: |
BJM
|
| País: |
Brazil
|
| Título: |
In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
|
| Autores: |
Zauli,Danielle Alves Gomes
Menezes,Carla Lisandre Paula de
Oliveira,Cristiane Lommez de
Mateo,Elvis Cristian Cueva
Ferreira,Alessandro Clayton de Souza
|
| Data: |
2016-12-01
|
| Ano: |
2016
|
| Palavras-chave: |
QPCR
Hepatitis B
Hepatitis C
|
| Resumo: |
Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400987
|
| Editor: |
Sociedade Brasileira de Microbiologia
|
| Relação: |
10.1016/j.bjm.2016.07.008
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Microbiology v.47 n.4 2016
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
