| Registro completo |
| Provedor de dados: |
58
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| País: |
Brazil
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| Título: |
Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
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| Autores: |
Nakanishi,Fernanda Akemi
Avila-Campos,Mario Julio
Kamiji,Nádia Hizuru
Itano,Eiko Nakagawa
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| Data: |
2006-03-01
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| Ano: |
2006
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| Palavras-chave: |
Immunoglobulin G
Actinobacillus actinomycetemcomitans
ELISA
Protease
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| Resumo: |
Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822006000100008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.37 n.1 2006
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| Direitos: |
info:eu-repo/semantics/openAccess
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