| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic Peninsula and its biotechnological potential in the production of fatty acids
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| Autores: |
Caamaño,Esteban
Loperena,Lyliam
Hinzpeter,Ivonne
Pradel,Paulina
Gordillo,Felipe
Corsini,Gino
Tello,Mario
Lavín,Paris
González,Alex R.
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| Data: |
2017-12-01
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| Ano: |
2017
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| Palavras-chave: |
Thraustochytrids
Antarctic
Docosahexaenoic acid
Eicosapentaenoic acid
Temperature
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| Resumo: |
ABSTRACT Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1-19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24-48% and 6.1-9.3%, respectively. All factors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10 °C and 25 °C). The growth in glucose at a temperature of 10 °C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400671
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1016/j.bjm.2017.01.011
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.48 n.4 2017
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| Direitos: |
info:eu-repo/semantics/openAccess
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