| Registro completo |
| Provedor de dados: |
Rev. Microbiol.
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| País: |
Brazil
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| Título: |
Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius
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| Autores: |
Silva,Claudio Henrique Cerri e
Puls,Jurgen
Sousa,Marcelo Valle de
Ferreira Filho,Edivaldo Ximenes
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| Data: |
1999-04-01
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| Ano: |
1999
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| Palavras-chave: |
Xylan
Xylanase
Aspergillus fumigatus
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| Resumo: |
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase activity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200005
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S0001-37141999000200005
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| Formato: |
text/html
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| Fonte: |
Revista de Microbiologia v.30 n.2 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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