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Provedor de dados:  Rev. Microbiol.
País:  Brazil
Título:  Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
Autores:  Maia,Maria de Mascena Diniz
Morais,Marcia Maria Camargo de
Morais Jr.,Marcos Antonio de
Melo,Eduardo Henrique Magalhães
Lima Filho,José Luiz de
Data:  1999-12-01
Ano:  1999
Palavras-chave:  Enzyme kinetics
Ester hydrolysis
Extracellular lipase
Fusarium solani
Resumo:  A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S0001-37141999000400003
Formato:  text/html
Fonte:  Revista de Microbiologia v.30 n.4 1999
Direitos:  info:eu-repo/semantics/openAccess
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