| Registro completo |
| Provedor de dados: |
Rev. Microbiol.
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| País: |
Brazil
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| Título: |
Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature
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| Autores: |
Carvalho,Patrícia de Oliveira
Oliveira,Joaquim Gilberto de
Pastore,Gláucia Maria
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| Data: |
1999-04-01
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| Ano: |
1999
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| Palavras-chave: |
Gamma-linolenic acid
Mucor sp
Unsaturated fatty acids
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| Resumo: |
As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production of GLA (74 mg/l) was obtained from the Mucor sp LB-54 strain after 5 days of incubation at 28°C in basal medium following glucose addition (7 % w/v) and incubation for an additional 3 days at 12°C. The identity of GLA found in the strain of Mucor sp LB-54 was confirmed by the coupled gas chromatography-mass spectrometry
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200016
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S0001-37141999000200016
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| Formato: |
text/html
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| Fonte: |
Revista de Microbiologia v.30 n.2 1999
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| Direitos: |
info:eu-repo/semantics/openAccess
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