| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil
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| Autores: |
Siqueira,Vera Lúcia Dias
Cardoso,Rosilene Fressatti
Pádua,Rubia Andreia Falleiros de
Caleffi-Ferracioli,Katiany Rizzieri
Helbel,Cesar
Santos,Adolfo Carlos Barreto
Aoki,Elisabeth Eyko
Nakamura,Celso Vataru
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| Data: |
2013-03-01
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| Ano: |
2013
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| Palavras-chave: |
Pseudomonas aeruginosa/antimicrobial resistance profile
Pseudomonas aeruginosa/genetic study
Acinetobacter spp./antimicrobial resistance profile
Acinetobacter spp./genetic study
Antimicrobial resistance
Bacterial typing
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| Resumo: |
In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Paraná, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-β-lactamase (MBL) was detected using a disk approximation test. Genotyping was performed using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Approximately 55% of the P. aeruginosa isolates and 92% of the Acinetobacter spp. isolates were multiresistant, but none were MBL-producers. ERIC-PCR revealed the presence of small clusters of carbapenem-resistant Acinetobacter spp., most likely OXA-type carbapenemase producers. Furthermore, high genetic diversity in P. aeruginosa and Acinetobacter spp. clinical isolates was observed, suggesting that cross-transmission is not very frequent in the studied hospital.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100006
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502013000100006
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.49 n.1 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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