| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Oral lactate intensifies insulin toxicity during severe insulin-induced hypoglycemia in mice
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| Autores: |
Vilela,Vanessa Rodrigues
Antunes,Marina Masetto
Godoi,Vilma Aparecida Ferreira
Travassos,Patricia Batista
Souza,Helenir Medri de
Bazotte,Roberto Barbosa
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| Data: |
2018-01-01
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| Ano: |
2018
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| Palavras-chave: |
Lactatemia
Oral glucose
Diazepam
Seizure
Anticonvulsant effect
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| Resumo: |
ABSTRACT We investigated whether oral lactate could prevent seizures and deaths in mice with severe hypoglycemia induced by a high dose of insulin. For this purpose, mice were fasted for 15 h and then given an intraperitoneal injection of regular insulin (5.0 U/kg or 10.0 U/kg). Immediately after insulin injection, the mice received an oral dose of saline (control), glucose (5.5 mmol/kg), or lactate (18.0 mmol/kg). Glucose and lactate levels were measured in the blood and brain before and after the seizures began. Glucose and lactate delayed (p < 0.05) the onset of seizures associated with severe insulin-induced hypoglycemia. Elevated (p < 0.05) brain levels of lactate were associated with an absence of seizures in mice that received glucose or lactate, suggesting that lactate could prevent convulsions associated with severe insulin-induced hypoglycemia. However, the same oral dose of lactate that delayed the onset of convulsions also increased the mortality rate. In contrast, diazepam (3.0 mg/kg) prevented seizures and markedly decreased the frequency of death during severe insulin-induced hypoglycemia. The results demonstrated that in contrast to oral glucose, oral lactate intensifies insulin toxicity.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200602
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/s2175-97902018000217617
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.54 n.2 2018
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| Direitos: |
info:eu-repo/semantics/openAccess
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