| Registro completo |
| Provedor de dados: |
BJPS
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| País: |
Brazil
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| Título: |
Production of intravenous human dengue immunoglobulin from Brazilian-blood donors
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| Autores: |
Gouveia,Frederico Leite
Oliveira,Renata Mendes Batista de
Lucena,Antônio Edson de Souza
Cordeiro,Marli Tenório
Leite,Ana Cristina Lima
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| Data: |
2013-12-01
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| Ano: |
2013
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| Palavras-chave: |
Viral infections
Dengue/treatment
Dengue/virus
Dengue/neutralization
Immunoglobulins/preparation
Plasma fractionation
Virus neutralization
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| Resumo: |
Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG collected from American blood donations was found to have low anti-dengue antibody titers. Overall, this anti-DENV IgG represents an important step in the study of the therapeutic potential and safety of a specific antibody that neutralizes the dengue virus in humans.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400022
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| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
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| Relação: |
10.1590/S1984-82502013000400022
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.49 n.4 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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